Lysozyme

Lysozyme is a 14kD enzyme directed against the b1 a 4 glycosidic bond between N-acetylglucosamine and Nacetylmuramic acid residues that make up peptidoglycan. Lysozyme is an antimicrobial protein secreted by polymorphonuclear leukocytes and is widely distributed in secretions such as airway secretions and nasal fluid whereas it is the most effective antimicrobial protein. It is also produced by monocytes, macrophages and epithelial cells. Lysozyme is able to kill bacteria by enzymatic lysis of bacterial cell walls and by a nonenzymatic mechanism.\n\nAllthough lysozyme is highly active against many gram-positive bacteria it is ineffective against gram-negative bacteria unless potentiated by certain cofactors (lactoferrin, antibody-complement or hydrogen peroxide-ascorbic acid). Next to its antimicrobial activity lysozyme has many other physiological functions including inactivation of certain viruses, important roles in surveillance of membranes of mammalian cells, immune regulatory activity, antiinflammatory and antitumor activity\n\nApplications:\nSuitable for use in Western Blot, Immunoassays, and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:10\nImmunoprecipitation: 1:10\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L9200-05D

Size

100ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Laible, N et al; Bactericidal activity of human lysozyme, muramidase inactivating lysozyme, and cationic polypeptides against Streptococcus sanguis and Streptococcus faecalis: inhibition by chitin oligosaccharides. Infect Immun 1985, 48: 720 2. Cole, A et al; Innate antimicrobial activity of nasal secretions. Infect Immun 1999, 67: 3267 3. Ibrahim, H et al; Strategies for new antimicrobial proteins and peptides: lysozyme and aprotinin as model molecules. Curr Pharm Des 2002, 8: 671 4. Cole, A et al; Cationic polypeptides are required for antibacterial activity of human airway fluid. J Immunol 2002, 169: 6985

Additional Info

Recognizes human lysozyme.