M-CSF Receptor, phosphorylated (Tyr699) (Macrophage-Colony Stimulating Factor Receptor, M-CSF, CSF-1)

Macrophage-colony stimulating factor (M-CSF, CSF-1) receptor is an integral membrane tyrosine kinase encoded by the c-fms proto-oncogene. M-CSF receptor is expressed in monocytes (macrophages and their progenitors) and drives growth and development of this blood cell lineage. (1-3). Binding of M-CSF to its receptor induces receptor dimerization, activation and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins (4). There are at least five major tyrosine autophosphorylation sites. Tyr723 (Tyr721 in mouse) is located in the kinase insert (KI) region. Phosphorylated Tyr723 binds the p85 subunit of PI3 kinase as well as PLCy2 (5). Phosphorylation of Tyr809 provides a docking site for Shc (5). Overactivation of this receptor can lead to a malignant phenotype in various cell systems (6). The activated M-CSF receptor has been shown to be a predictor of poor outcome in advanced epithelial ovarian carcinoma (7) and breast cancer (8). \n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS,\n0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137464

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and <0.02% sodium azide.

P Type

Mab

Purity

Purified.

Isotype

IgG

References

1. Stanley, E. R. et al. (1978) Nature 274, 168-170. 2. Byrne, P. V. et al. (1981) J. Cell. Biol. 91, 848-853.\n3. Bourette, R. P. et al. (2000) Growth Factors 17, 155-166. 4. Novak, U. et al. (1996) Oncogene 13, 2607-2613. 5. Bourette, R. P. et al. (1997) EMBO J. 16, 5880-5893. 6. Morley, G. M. et al. (1999) Oncogene 18, 3076-3084. 7. Toy, E. P. et al. (2001) Gynecol. Oncol. 80, 194-200. 8. Maher, M. G. et al. (1998) Clin. Cancer Res. 4, 1851-1856. 9. Hamilton, J.A. (1997) J Leukoc Biol 62, 145-55. 10. Downing, J.R. et al. (1991) Mol Cell Biol 11, 2489-95.

Additional Info

Recognizes endogenous levels human M-CSF Receptor only when phosphorylated at Tyr699. May cross-react with other activated tyrosine kinases including PDGF receptors and EGFR.