Macrophage (L1 Protein, Calprotectin, Cystic Fibrosis Antigen)

L1 Protein or Calprotectin Molecule is an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and by tissue macrophages.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions:\nImmunohistochemistry (Formalin/paraffin): 1:1000 for 20 minutes at RT. For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS for 10 minutes at 37 degrees C.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Positive Control: \nTonsil, lymph node or spleen\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M1202-74

Size

500ul

Applications

IHC

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can, Fel, GP, NHP, Prc

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and sodium azide. See M1202-74X.

P Type

Mab

Purity

Purified by Protein G affinity chromatography

Isotype

IgG1

References

1.

Additional Info

Recognizes human Macrophage. Reacts with neutrophils, monocytes and squamous mucosal epithelia. Has been shown as an excellent marker in tissue sections. Species Crossreactivity: baboon, monkey, porcine, feline, canine, guinea pig, rat and mouse.