Macrophage (L1 Protein, Calprotectin, Cystic Fibrosis Antigen)

The L1 or Calprotectin molecule is an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.\n\nApplications:\nSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 1:50-1:100; 10ul lables 1x10e6 cells in 100ul. Membrane permeabilization is required for this application.\nImmunohistochemistry (Frozen and Paraffin): 1:100-1:200; Prior to staining paraffin sections, product requires protein digestion pre-treatment e.g. trypsin, 0.1% for 10 minutes or antigen retrieval using heat treatment. Frozen sections do not require pretreatment.\nOptimal working dilutions to be determined by researcher.\n\nRecommended Positive Control:\nHuman spleen\n\nRecommended Negative Control: \nI1904-78R: IgG1: Murine Negative Control\n\nRecommended Secondary Antibodies:\nI1904-65W: IgG, F(ab

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SPECIFICATIONS

Catalog Number

M1202-76

Size

200ug

Applications

FC, IHC

Hosts

Mouse

Reactivities

Hum, Rat, Bov, Can, Fel, Goat, GP, NHP, Prc, Rabbit

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

US Biological application reference: Grosche, A. et al., (2013) Am. J. Veterinary Research 74; 1281-1290. 1. Brandtzaeg, P., et al., J. Clin. Path. 41: 963-970 (1988). 2. Brandtzaeg, P., et al., Histopathol. 21: 191-196 (1992). 3. Flavell, D.J., Jones, D.B. and Wright, D.H., J. Histochem. Cytochem. 35: 1217-1226 (1987). 4. Gutierrez, M., et al., Vet. Immunol. Immunopathol. 71: 321-334 (1999). 5. Ramsay, A.D., et al., Journal of Pathology 164: 321-328 (1991). 6. Christgau, M., et al., Journal of Histochemistry & Cytochemistry 46(12): 1443-1454 (1998). 7. Perez, J., et al., J. Comp. Path. 121: 385-397 (1999). 8. Obert, L., et al., J. Virol. 76(12): 6311-6322 (2002). 9. Malik, N., et al., Circulation 98: 1657-1665 (1998). 10. Bagavant, H., et al., Am. J. Pathol. 160: 141-149 (2002).

Additional Info

Recognizes human L1 or Calprotectin molecule. Species Crossreactivity: Rhesus and Cynomolgus monkey, baboon, feline, canine, guinea pig, equine, rabbit, rat, goat, porcine, bovine and Fallow deer.