Major Basic Protein (MBP)

Applications: \nSuitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (fresh frozen): Tissues were sectioned (6um), dried for 1 hour at RT and fixed in 1:1 solution of methanol and acetone. Sections were air dried for 1 hour. Sections may be stored wrapped in aluminium foil at -70 degrees C until used. \nImmunohistochemistry (Paraffin): 4% PFA (3hr) should be used for skin biopsies. 5um sections are dewaxed and rehydrated with water. A proteinase treatment of 0.1% trypsin for 15 minutes in water should be performed prior to blocking and antibody staining.\nNote: The antibody reacts better in frozen tissues than in paraffin embedded tissues.\nWestern Blot: Purified normal human eosinophils were treated with digitonin (5mg/ml) for 10 minutes before boiling in sample buffer. Ran on a 15% SDS-PAGE. Purified MBP migrates at ~14kD, with typical human samples ranging from 10-16kD.\nOptimal dilutions to be determined by the researcher.\n\nSource: \nTissue culture supernatant \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M2110

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 10mM sodium azide, before the addition of glycerol to 40%.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Ying, S., et al., ACI International 14: 64-71 (2002). 2. Moqbel, R., et al., Clin. Exp. Allergy 22: 265-273 (1992). 3. Kroegel, C., et al., Lung 168: (Suppl) 5-17 (1990).

Additional Info

Recognizes human eosinophil major basic protein independent of the stages of eosinophil activation and can be regarded as a pan-eosinophil marker. Recognizes the 28.3kD secreted pro-form of MBP (Pregnancy Associated MBP) found in the placenta and serum of pregnant women as well as the 13.8kD mature form of the protein which is found in the matrix of the eosinophil large specific granual. Species Crossreactivity: Does not crossreact with equine, mouse or rat MBP.