Malate Dehydrogenase (MDH)

Size

10mg

Hosts

Sheep

Form

Supplied in PBS, pH 7.2, 0.01% sodium azide, 40% glycerol.

Purity

Puri?ed by delipidation, salt fractionation and ion exchange chromatography.

References

1. Asahi, T., Nishimura, M., Regulatory Function of Malate Dehydrogenase Isoenzymes in the Cotyledons of Mung Bean. J. Biochem. 73: 217 (1973). 2. Baker, B., Bramhall, R., Irreversible Enzyme Inhibitors. 189. Inhibition of Some Dehydrogenases by Derivatives of 4-Hydroxyquinoline-2- and -3-Carboxylic Acids. J. Med. Chem. 15: 230 (1972). 3. Banaszak, L., Bradshaw, R., , The Enzymes, 3rd Ed. 11 , P. Boyer, Academic Press, NY, 369 (1975). 4. Beaton, J., Liver and Plasma Malic Dehydrogenase Activities in the Exercised Rat, Proc. Soc. Exp. Biol. Med. 123: 598 (1966). 5. Berkes-Tomasevic, P., Holzer, H., Zur Regulation der Synthese der Malatdehydrogenasen aus Cytosol und Mitochondrein von Rattenleber, Eur. J. Biochem. 2: 98 (1967). 6. Bleile, D., Foster, M., Brady, J., Harrison, J., Identification of Essential Arginyl Residues in Cytoplasmic Malate Dehydrogenase with Butanedione, J. Biol. Chem. 250: 6222 (1975). 7. Blonde, D., Kresack, E., Kosicki, G., The Effects of Ions and Freeze-Thawing on the Supernatant and Mitochondrial Malate Dehydrognase, Can. J. Biochem. 45: 641 (1967). 8. Callahan, J., Kosicki, G., The Effect of Lipid Micelles on Mitochondrial Malate Dehydrogenase, Can. J. Biochem. 45: 839 (1967). 9. Cassman, M., King, R., Molecular Weight Studies on the Supernatant Malate Dehydrogenase Isolated from Beef Heart. Evidence for a Proteolytic Contaminant in the Purified Preparation, Biochim. Biophys. Acta 257: 143 (1972). 10. Cassman, M., King, R., Subunit Interactions and Ligand Binding in Supernatant Malic Dehydrogenase. Cooperative Binding of Reduced Nicotinamide Adenine Dinucleotide Associated with a Monomer-Dimer Equilibrium of the Proton, Biochem. 11: 4937 (1972). 11 Cassman, M., Beef Heart Malic Dehydrogenase. VI. A pH-Dependent Transition Observed in the Presence of Reduced Diphosphopyridine Nucleotide, J. Biol. Chem. 242: 2013 (1967). 12. Cennamo, C., Montecuccoli, G., Konig, G., Inhibition of Mitochondrial Malic Dehydrogenase by Citrate, Biochim. Biophys. Acta 139: 514 (1967). \n Additional references:\nChen, S., and Engel, P.: Reversible Modification of Pig Heart Mitochondrial Malate Dehydrogenase by Pyridoxal 5'-Phosphate, Biochem J 151 , 297, 1975 \n \nCodd, G.: The Photoinhibition of Malate Dehydrogenase, FEBS Lett 20 , 211, 1972 \n \nCurry, R., and Ting, I.: Purification and Crystallization of Three Isoenzymes of Malate Dehydrogenase From Zea mays Seed, Arch Biochem Biophys 158 , 213, 1973 \n \nDavidson, R., and Cortner, J.: Mitochondrial Malate Dehydrogenase: A New Genetic Polymorphism in Man, Science 157 , 1569, 1967 \n \nde Jong, D., and Olson, A.: Electrophoretic Migration and Redox Behavior of Malate Dehydrogenases From Cell Suspension Cultures of Tobacco, Biochim Biophys Acta 276 , 53, 1972 \n \nDeArriaga, D., Teixido, F., Busto, F., and Soler, J.: The Nature of the Substrate Inhibition of Cytoplasmic Malate Dehydrogenase from Phycomyces blakesleeeanus ,Biochim Biophys Acta 784 , 158, 1984 \n \nDevenyi, T., Rogers, S., and Wolfe, R.: Structural Studies of Pig Heart Malate Dehydrogenase, Nature 210 , 489, 1966 \n \nDodd, G.: The Interaction of Glutamate Dehydrogenase and Malate Dehydrogenase with Phospholipid Membranes, Eur J Biochem 33 , 418, 1973 \n \nDomenech, D., Abante, J., Bozal, F., Mazo, A., Cortes, A., and Bozal, J.: An Improved Purification Method for Cytosolic Malate Dehydrogenase from Several Sources, Prep Biochem 18 , 17, 1988 \n \nDubery, I., and Schabort, J.: Purification and Characterization of NADP-linked Malate Dehydrogenase (Decarboxylating) from Mangifera indica ,Biochim Biophys Acta 662 , 102, 1981 \n \nDuVal, G., Swaisgood, H., and Horton, H.: Some Kinetic Characteristics of Immobilized Protomers and Native Dimers of Mitochondrial Malate Dehydrogenase: An Examination of the Enzyme Mechanism, Biochem 24 , 2067, 1985 \n \nEberhardt, N., and Wolfe, R.: Malate Dehydrogenase. Circular Dichroism Difference Spectra of Porcine Heart Mitochondrial and Supernatant Enzymes. Binary Enzyme-Coenzyme, and Ternary Enzyme-Coenzyme-Substrate Analog Complexes, J Biol Chem 250 , 2987, 1975 \n \nFerguson, J., Boll, M., and Holzer, H.: Yeast Malate Dehydrogenase: Enzyme Inactivation in Catabolite Repression, Eur J Biochem 1, 21, 1967 \n \nFickenscher, K., and Scheibe, R.: Purification and Properties of NADP-Dependent Malate Dehydrogenase from Pea Leaves, Biochim Biophys Acta 749 , 249, 1983 \n \nFlury, U., Heer, B., and Feichter, A.: Regulatory and Physicochemical Properties of Two Isoenzymes of Malate Dehydrogenase from Schizosaccharomyces pombe ,Biochim Biophys Acta 341 , 465, 1974 \n \nFoster, M., and Harrison, J.: Characterization of Porcine Malate Dehydrogenase. II. Amino Acid Sequence of a Peptide Containing the Active Center Histidine Residue, Biochim Biophys Acta 351 , 295, 1974 \n \nFrieden, C., and Fernandez-Sousa, J.: Kinetic Studies on Pig Heart Cytoplasmic Malate Dehydrogenase, J Biol Chem 250 , 2106, 1975 \n \nFriedman, M., and Teggins, J.: The Blocking of the Tetrachloroplatinate (II) Inhibition of Malate Dehydrogenase by Sulfur-Containing Amino Acids, Biochim Biophys Acta 341 , 277, 1974 \n \nFriedman, M., Otwell, H., and Teggins, J.: Protection of the Active Site of Mitochondrial Malate Dehydrogenase from Inhibition by Potassium Tetrachloroplatinate, Biochim Biophys Acta 391 , 1, 1975 \n \nGelpi, J., Dordal, A., Montserrat, J., Mazo, A., and Cortes, A.: Kinetic Studies of the Regulation of Mitochondrial Malate Dehydrogenase by Citrate, Biochem J 283 , 289, 1992 \n \nGeorge, H., McMahan, J., Bowler, K., and Elliott, M.: Stabilization of Lactate and Malate Dehydrogenase by Organic Solvents, Biochim Biophys Acta 191 , 466, 1969 \n \nGlatthaar, B., Banaszak, L., and Bradshaw, R.: The Identification of an Asymmetric Complex of Nicotinamide Adenine Dinucleotide and Pig Heart Cytoplasmic Malate Dehydrogenase, Biochem Biophys Res Commun 46 , 757, 1972 \n \nGleason, W., Fu, Z., Birktoft, J., and Babaszak, L.: Refines Crystal Structure of Mitochondrial Malate Dehydrogenase from Porcine Heart and the Concensus Structure for Dicarboxylic Acid Oxidoreductases, Biochemistry 33 , 2078, 1994 \n \nGregory, E.: Chemical Modification of Bovine Heart Mitochondrial Malate Dehydrogenase. Selective Modification of Cysteine and Histidine, J Biol Chem 250 , 5470, 1975 \n \nGutman, M., and Hartstein, E.: Inhibition of Mitochondrial Malate Dehydrogenase by 2-Thenolytrifluoroacetone, FEBS Lett 49 , 170, 1974 \n \nHall, M., Levitt, D., and Banaszak, L.: Crystal Structure of Escherichia coli Malate Dehydrogenase - A Complex of the Apoenzyme and Citrate at 1.87-Angstrom Resolution, J Mol Biol 226 , 867, 1992 \n \nHayashi, M., and Unemoto, T.: The Presence of D-Malate Dehydrogenase (D-Malate:NAD Oxidoreductase) in Serratia marcesceus ,Biochim Biophys Acta 122 , 374, 1966 \n \nHayden, D., and Cook, F.: Malate Dehydrogenase in Maize Endosperm; the Intracellular Location and Characterization of the Two Major Particulate Isoenzymes, Can J Biochem 50 , 663, 1972 \n \nHill, E., Tsernoglou, D., Webb, L., and Banaszak, L.: Polypeptide Conformation of Cytoplasmic Malate Dehydrogenase from an Electron Density Map at 3.0