MALT1, CT (MLT1, Mucosa-associated lymphoid tissue lymphoma translocation protein 1, Paracaspase 1)

Mucosa-associated lymphoid tissue translocation gene 1 (MALT1) is a paracaspase that is a critical mediator of T-cell receptor activation of NF-kappaB and may contribute to the progression of MALT lymphomas (1-4). It contains two immunoglobulin-like domains, an amino-terminal death domain and a carboxy-terminal caspase-like domain. Association of MALT1 with Bcl-10 and CARD11/Carma1 leads to activation of IKK and subsequent stimulation of NF-kappaB, resulting in increased proliferation and inhibition of apoptosis (5,6). A common translocation in MALT B-cell non-Hodgkin lymphomas t(11;18)(q21;q21) results in the fusion of the amino terminus of API2 (c-IAP2), a member of the inhibitor of apoptosis protein family, to the carboxy terminus of MALT1 (1,2). The API2-MALT1 fusion protein likely leads to deregulation of NF-kappaB, contributing to increased oncogenic potential (7).\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunoprecipitation: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M2148-01

Size

100ul

Applications

ELISA, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A and peptide affinity chromatography.

Isotype

IgG

References

1. Akagi, T. et al. (1999) Oncogene 18, 5785-5794. 2. Uren, A.G. et al. (2000) Mol. Cell 6, 961-967. 3. Ruland, J. et al. (2003) Immunity 19, 749-758. 4. Nakagawa, M. et al. (2006) Leukemia 20, 929-936. 5. Che, T. et al. (2004) J. Biol. Chem. 279, 15870-15876. 6. Lucas, P.C. et al. (2001) J. Biol. Chem. 276,\n19012-19019. 7. Ho, L. et al. (2005) Blood 105, 2891-2899.

Additional Info

Recognizes endogenous levels of total mouse MALT1 at ~90kD. Species Crossreactivity: Human, rat