MAML1 (Mastermind-like Protein 1)

Mastermind-like (MAML) family of proteins are homologs of Drosophila Mastermind. The family is composed of three members in mammals: MAML1, MAML2, and MAML3 (1,2). MAML proteins form complexes with the intracellular domain of Notch (ICN) and the transcription factor CSL (RBP-Jk) to regulate Notch target gene expression (3-5). MAML1 also interacts with myocyte enhancer factor 2C (MEF2C) to regulate myogenesis (6). MAML2 is frequently found to be fused with Mucoepidermoid carcinoma translocated gene 1 (MECT1, also know as WAMTP1 or TORC1) in patients with mucoepidermoid carcinomas and Warthin's tumors (7).\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:200\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137466

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and <0.02% sodium azide.

P Type

Mab

Purity

Purified.

Isotype

IgG

References

1. Wu, L. et al. (2002) Mol. Cell Biol. 22, 7688-7700. 2. Lin, S.E. et al. (2002) J. Biol. Chem. 277, 50612-50620. 3. Kitagawa, M. et al. (2001) Mol. Cell Biol. 21, 4337-4346. 4. Nam, Y. et al. (2003) J. Biol. Chem. 278, 21232-21239. 5. Nam, Y. et al. (2006) Cell 124, 973-983. 6. Shen, H. et al. (2006) Genes Dev. 20, 675-688. 7. Tonon, G. et al. (2003) Nat. Genet. 33, 208-213.

Additional Info

Recognizes endogenous levels of total human MAML1. Species Crossreactivity: mouse, rat. Does not detect MAML2 or MAML3.