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Mannan Binding Protein (MBP, Mannose Binding Protein, Mannan Binding Lectin, MBL)

Cat no: M2245

Mannan Binding Protein (MBP, Mannose Binding Protein, Mannan Binding Lectin, MBL)

This gene encodes the soluble mannose-binding lectin or mannose-binding protein found in serum. The protein encoded belongs to the collectin family and is an important element in the innate immune system. The protein recognizes mannose and N-acetylglucosamine on many microorganisms, and is capable of activating the classical complement pathway. Deficiencies of this gene have been associated with susceptibility to autoimmune and infectious diseases.\n\nApplications: \nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:5000-1:10,000. Reacts with the ~30kD human MBL protein. Suggested blocking and dilution buffer is TBST with 0.05% Tween-20, 5% skim milk. Suggested incubation time is one hour at RT.\nELISA (Direct and indirect): 1:5000-1:10,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M2245

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4.

P Type

Mab

Purity

Ascites

Isotype

IgG1

Additional Info

Recognizes both natural and recombinant human Mannan (Mannose)-Binding Lectin (MBL) protein.

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