Mannose Receptor (MR, CD206)

The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180kD transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells. The expression of MR on monocytes increases during culture and can be enhanced by cytokines as IFN-g. Labeling of MR expressing monocytes/macrophages increases at 37 degrees C with prolonged incubation time probably due to internalization of the MR-antibody-complex. The antibody prevents binding of glycoproteins including t-PA to MR. Detection of the MR with anti-MR monoclonal antibody can substitute staining for mannose containing probes as labeled mannosylated BSA, a technique which is more cumbersome and less specific.\n\nApplications:\nSuitable for use in Immunohistochemistry, Western Blot, Flow Cytometry and functional studies. Other applications have not been tested.\n\nRecommended Dilution:\nImmunohistochemistry (frozen sections): 1:50, fixed in acetone/chloroform\nFlow Cytometry: 1:50\nWestern Blot: 1:50 (non-reduced sample, 6% SDS-PAGE)\nFunctional Studies: M2256-01 functions as an inhibitor of binding. The antibody was functionally tested by preincubation of immobilized receptor with antibody. This prevented binding of t-PA to immobilized receptor (1).\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: macrophages\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M2256-01

Size

100ug

Applications

FC, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, containing 0.02% sodium azide and 0.1% BSA.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Barret-Berghoeff, M et al; Monoclonal antibodies against the human mannose receptor that inhibit the binding of tissue-type plasminogen activator. Thromb Haemostas 1997, 77: 718 2. Noorman, F et al; Monoclonal antibodies against the human mannose receptor as a specific marker in flow cytometry and immunohistochemistry for macrophages. J Leukocyte Biol 1997, 61: 63 3. Noorman, F et al; Cluster Mannosides can inhibit mannose receptor-mediated tissue-type plasminogen activator degradation by both rat and human cells. Hepatology 1997, 26: 1303.

Additional Info

Recognizes human Mannose Receptor at ~175kD.