MAP-2 (Microtubule-associated Protein 2, Map2, Mtap2) (Biotin)

The microtubule-associated protein 2 (MAP-2) is an abundant neuronal cytoskeletal protein that binds to tubulin and stabilizes microtubules. MAP-2 is essential for the development and maitenance of neuronal morphology. Three primary isoforms of MAP-2, the high molecular weight MAP2a and MAP2b, and the low molecular weight MAP2c (resulting from alternate splicing of the MAP2 gene), have been identified in neurours. The low molecular weight isoform, MAP2c, is expressed in developing brain and is down-regulated during brain maturation, whereas the high molecular weight MAP2b is expressed in both developing and adult brain. The MAP2a appears only after brain maturation. All these forms bind to microtubules through a domain near the carboxyl terminus that contains either three or four similar repeats of a 31aa motif. MAP-2 together with MAP-4 and tau proteins belong to the family of thermostable proteins associated with microtubules.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: 1:100 \nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nAdult mouse brain tissue\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

029533

Size

100ul

Applications

IHC

Hosts

Rabbit

Reactivities

Mouse, Rat

Conjugates

Biotin

Form

Supplied as a liquid in PBS, 0.1% sodium azide, 15mg/ml BSA. Labeled with Biotin.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Matsuda, K., et al. (2006). J Biol Chem. 281: 25577-25587. 2. Yasuhara, T., et al. (2006). J. Neurosci. 26: 12497-12511. 3. Sigurjonsson, O., et al. (2005). Proc Natl Acad Sci USA. 102:5227-5232. 4. Tan, P., et al. (2007). Proc. Natl. Acad. Sci. USA. 104:17524-17529. 5. Huang, C., et al. (2008). J Neurosci Res. 86(6): 1277-1289.

Additional Info

Recognizes rat MAP-2. Species Crossreactivity: Mouse.