MAP Kinase 1 (Mitogen-activated Protein Kinase 1, MAPK 1, MAPK1, ERT1, Extracellular Signal-regulated Kinase 2, ERK2, ERK-2, ERK, MAP Kinase Isoform p42, Mitogen-activated Protein Kinase 2, MAP Kinase 2, MAPK 2, MAPK2, p41mapk, p42-MAPK, P42MAPK, PRKM1, P

Cell proliferation is regulated in several contexts, for example during development, tissue differentiation, wound healing and immune responses. In mammalian cells, proliferative signals lead to the activation of a protein kinase cascade, resulting in the phosphorylation of two closely related Mitogen-Activated Protein Kinases (MAPKs) ERK1 and ERK2. When activated, ERKs form dimers that translocate to the nucleus where they phosphorylate several classes of transcription factors which are involved in the up-regulation of immediate early genes. As such, Erk1 and Erk2 represent a paradigm for a growing family of proline-directed protein kinases that mediate entry, progression and exit from the cell cycle in diverse eukaryotic cells. These enzymes function within highly conserved cascade of sequentially activating protein kinases that transduce\nsignals from diverse extracellular stimuli. In vertebrates the mitogen-induced sequential activation of the kinases Raf1->Mek1->Erk2->Rsk occurs via the G-protein Ras.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\nOptimal dilutions to be determined by the researcher.\n\nPositive controls: U937, HeLa, NIH-3T3, RAW\n264.7, LNCaP and HEK whole cell lysates.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M2351-80Y

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as liquid in PBS, 0.1% sodium azide. No stabilizing proteins added.

P Type

Pab

Purity

Serum

References

Pelech, SL and Charest, DL (1995) MAP kinase-dependent pathways in cell cycle control. Prog Cell Cycle Res 1(11):33-52.\nGarrington, TP and Johnson, GL (1999) Organization and regulation of mitogen-activated protein kinase signaling pathways.\nCurr Opin Cell Biol 11(2) Apr, 211-218.\nElion, EA (1998) Routing MAP kinase cascades. Science 281(5383):1625-1626.\nAsthagiri, AR et al. (2000) The role of transient ERK2 signals in fibronectin- and insulin-mediated DNA synthesis. J. Cell Sci.\n113 (24) 2000 4499-4510 .\nHoffmann, R. et al. (1999) The MAP kinase ERK2 inhibits the cyclic AMP-specific phosphodiesterase HSPDE4D3 by\nphosphorylating it at Ser579. EMBO J. 18, 893-903.

Additional Info

Recognizes Human Erk-2 at 42kD. Species Crossreactivity: human and mouse. Species sequence homology: rat sequence differs by a single, conservative amino acid substitution.

Alternative Names

EC=2.7.11.24, p38, p40, p41