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MAP Kinase 2 (Mitogen-activated Protein Kinase 2, MAPK 2, MAPK2, Extracellular Signal-regulated Kinase 2, ERK2, ERK-2, ERK, ERT1, MAP Kinase Isoform p42, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, Mapk1, Mapk, p42mapk, p42-MAPK, Prkm1, PRKM

Cat no: M2362

MAP Kinase 2 (Mitogen-activated Protein Kinase 2, MAPK 2, MAPK2, Extracellular Signal-regulated Kinase 2, ERK2, ERK-2, ERK, ERT1, MAP Kinase Isoform p42, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, Mapk1, Mapk, p42mapk, p42-MAPK, Prkm1, PRKM

ERK2 is activated by MEK2, which phosphorylates neighboring threonine 183 and tyrosine 185 residues. ERK2 is a serine/threonine kinase that phosphorylates MAP2 and myelin basic protein. This kinase is an important proximal component of the MAP kinase pathway involved in transeitting the signals from growth factors, neurotransmitters and hormones at the cell surface to the transcriptional events in the nucleus.\n\nApplications: \nSuitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nImmunoprecipitation: 4ug immunoprecipitates MAP Kinase from a RIPA lysate from mouse 3T3 cells. Boil the cell lysate and let cool before adding the antibody as this antibody only binds to denatured MAP Kinase.\nWestern Blot: 0.5-2ug/ml detects MAP Kinase in RIPA lysates from human A431 carcinoma cells. A non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with M2362 (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody (HRP) and a chemiluminescence detection system. A previous lot detected MAP Kinase in mouse 3T3 fibroblasts and rat L6 cells.\nOptimal dilutions to be determined by the researcher.\n\nIncluded Positive Control: \nA0001-50: Non-stimulated A431 cell lysate. Add 2.5ul of 2-mercaptoethanol per 100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M2362

Size

200ug

Applications

ELISA, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography

Isotype

IgG2a

References

1. Boulton, T.G., et al., Science 249: 64

Additional Info

Recognizes mouse MAP Kinase encoded by the mapk gene at ~42kD. Species Crossreactivity: human and rat

Alternative Names

9030612K14Rik, AA407128, AU018647, C78273, EC=2.7.11.24, p41mapk

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