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MAP Kinase 3 (Mitogen-activated Protein Kinase 3, MAPK 3, Mapk3, ERT2, Extracellular Signal-regulated Kinase 1, ERK1, ERK-1, Esrk1, Insulin-stimulated MAP2 Kinase, MAP Kinase Isoform p44, Microtubule-associated Protein 2 Kinase, Mitogen-activated Protein

Cat no: M2360-02S

MAP Kinase 3 (Mitogen-activated Protein Kinase 3, MAPK 3, Mapk3, ERT2, Extracellular Signal-regulated Kinase 1, ERK1, ERK-1, Esrk1, Insulin-stimulated MAP2 Kinase, MAP Kinase Isoform p44, Microtubule-associated Protein 2 Kinase, Mitogen-activated Protein

Erk1/extracellular signal-regulated kinase 1/p44 MAP kinase/microtubule-associated protein 2 kinase/myelin basic protein kinase/EGF receptor T669 kinase is ubiquitously expressed in various tissues and cells. It gets activated by phosphorylation of neighboring serine and threonine residues by Mek1 and Mek2 in response to diverse stimuli like growth factors and mitogens.\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilution: \nWestern Blot: 2-4ug/ml for 2 hrs at RT.\nImmunohistochemistry (Formalin/paraffin): 1:25-1:50 for 30 min. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.\nOptimal dilutions to be determined by the researcher. \n\nPositive Control: \nA431 cells. Breast.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M2360-02S

Size

250ul

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.6, 0.2% BSA, 15mM sodium azide, before the addition of glycerol to 40%.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes rat Erk1. Species Crossreactivity: Human and mouse.

Alternative Names

EC=2.7.11.24

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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