MAP Kinase Kinase 3 (Dual Specificity Mitogen-activated Protein Kinase Kinase 3, Mitogen-activated Protein Kinase Kinase 3, MAP2K3, MAPKK 3, MKK3, MAPK/ERK Kinase 3, MEK 3, MEK3, PRKMK3, Stress-activated Protein Kinase Kinase 2, SAPK Kinase 2, SAPKK2, SAP

MKK3 is a protein kinase that phosphorylates the p38 MAPK but not ERK MAPK. Phosphorylation by MKK3 occurs on threonine and tyrosine residues and increases the activity of p38 to stimulate transcription factors ATF2 and Elk-1. MKK3, together with MKK6, serve as upstream regulators of p38 MAPK activation. A structural variant, MKK3b, has been identified that contains 29 more amino acids at its N-terminus.\n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:2000\nImmunoprecipitation: 1:2000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M2363-10B

Size

200ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder in 0.1% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

References

1. Berijard, B. et al. (1995) Independent human MAP-kinase signal transduction pathways defined by MEK and MKK isoforms. Science 267:682-5. 2. Raingeaud, J. et al. (1996) MKK3- and MKK6-regulated gene expression is mediated by the p38 mitogen-activated protein kinase signal transduction pathway. Mol Cell Biol. 16:1247-55. 3. Moriguchi, T. et al. (1996) A novel kinase cascade mediated by mitogen-activated protein kinase kinase 6 and MKK3. J. Biol. Chem. 271:13675-9. 4. Han, J. et al. (1997) Identification and characterization of a predominant isoform of human MKK3. FEBS Lett. 403:19-22.

Additional Info

Reacts with human MKK3.