MAP Kinase Kinase 5 (Dual Specificity Mitogen-activated Protein Kinase Kinase 5, MAP2K5, MAPKK 5, MAPKK5, MKK5, PRKMK5, HsT17454, MAPK/ERK Kinase 5, MEK 5, MEK5)

Mitogen-acitvated protein kinase kinase 5 (MKK5) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MAPKs require dual phosphorylation on threonine and tyrosine residues, an activation step carried out by MAPK kinase (1-2). The MAPK cascade plays an important role in signaling defense responses. Of the MKK family members, MKK5 exhibits the closest homology to MEK1 and MEK2, with 46% amino acid identity in the catalytic domain (3) MKK has been show to activate extracellular-signal-regulated protein kinase 5 (ERK5). Also, activated ERK5 phosphorylates mitogen- MKK5 extensively at Ser129, Ser137, Ser142 and Ser149, which are located within the region in MKK5 that is thought to interact with ERK5 (3). There have also been findings to that the MEK5 (MKK5)/ERK5 (BMK) pathway may play a role in prostate cancer (5). \n \nApplications: \nSuitable for use in Immunocytochemistry, Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:10,000 \nImmunocytochemistry: 1:100-1:250 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

M2363-19B

Size

100ul

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Han J, et al. J Bio Chem 271:2886-2891, 1996 2. Seger R, et al. FASEB J. 9:726-735, 1995 3. Mody N, et al. FEBS Lett 502:21-24, 2001 4. Mody N, et al. J. Bio Chem 372:567-575, 2001 5. Mehta PB, et al. Oncogene 22:1381-1389, 2003

Additional Info

Recognizes human MKK5.

Alternative Names

Mitogen-activated Protein Kinase Kinase 5, EC=2.7.12.2