MAP Kinase Kinase 6, CT (Dual Specificity Mitogen-activated Protein Kinase Kinase 6, MAP2K6, MAPKK 6, MAPKK6, MKK6, PRKMK6, MAPK/ERK Kinase 6, MEK 6, MEK6, Stress-activated Protein Kinase Kinase 3, SAPK Kinase 3, SAPKK3, SAPKK-3, SKK3)

Mitogen-acitvated protein kinase kinase 6 (MKK6) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MAPKs require dual phosphorylation on threonine and tyrosine residues, an activation step carried out by MAPK kinase (1-2). MKK6, closely related to MKK3, efficiently phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines and environmental stress but not JNK or ERK (3). MTK1 phosphorylates MKK6 on serine 207 and threonine 211 (4). \n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:10,000 \nImmunohistochemistry: 1:100-1:250 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

M2363-22F

Size

100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Han J et al. J. Biol. Chem. 271: 2886-2891, 1996. 2. Seger, R.; Krebs, EG. : FASEB J. 9: 726-735, 1995. 3. Bernd S et al. 271(19); 11427-11433, 1997. 4. Hiroaki M et al. Mol. Cell. Biol. 22(13):4544-4555, 1999.

Additional Info

Recognizes human MKK6. Species crossreactivity: mouse and rat.

Alternative Names

Mitogen-activated Protein Kinase Kinase 6, EC=2.7.12.2