MAP Kinase Kinase 7 (Dual Specificity Mitogen-activated Protein Kinase Kinase 7, MAP2K7, MAPKK 7, MAPKK7, MKK7, PRKMK7, c-Jun N-terminal Kinase Kinase 2, JNK Kinase 2, JNKK 2, JNKK2, JNK-activating Kinase 2, MAPK/ERK Kinase 7, MEK 7, MEK7, Stress-activate

Mitogen-activated protein kinase (MAP kinase) signal transduction pathways have been implicated in multiple physiological and pathophysiological processes including growth differentiation, survival and death. The p38 group of MAP kinases is activated by treatment of cells with proinflammatory cytokines and by exposure to environmental stress. One important\nfunction of the p38 signaling pathways appears to be the regulation of cytokine expression. The MAP kinases are activated by conserved protein kinase signaling modules, which include a MAP kinase kinase kinase (MKKK) and a dual-specificity MAP kinase kinase (MKK). The MKKK phosphorylates and activates the MKK, which, in turn, activates the MAP kinase by dual phosphorylation on threonine and tyrosine residues within a Thr-Xaa-Tyr motif located in protein kinase subdomain VIII. MKK7 is a novel activator of c-Jun N-terminal kinase (JNK). This 47-Kda protein is widely expressed in human and mice. The kinase domain of MKK7 is closely related to a Drosophila JNK kinase dHep (69% homology) and C. elegans (54% homology), and it is mildly related to MKK4, MKK3, and MKK6. MKK7 phosphorylates and activates JNK1 in vitro.\n\nApplications:\nSuitable for use in Immunofluorescence, Western Blot and ELISA. Other applications have not been tested.\n\nRecommended Dilution:\nImmunofluorescence: 15-20ug/ml\nWestern Blot: 1-3ug/ml\nELISA: 0.1-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M2363-95G

Size

100ug

Applications

ELISA, IF, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.4, containing 0.1% sodium azide

P Type

Mab

Purity

Purified by Protein A chromatography

Isotype

IgG1

Additional Info

Reacts with the ~47kD human and mouse MKK7 protein. There is no cross reactivity to MKK4. Reactivity is confirmed with MKK7 transfected COS cells, MDCK, mouse liver and Caco2 cell lysates. MDCK is the recommended positive control for this antibody.