MAP Kinase p38 alpha (Mitogen-activated Protein Kinase p38 alpha, MAPK p38 alpha, Cytokine Suppressive Anti-inflammatory Drug-binding Protein, CSAID-binding Protein, CSBP, CSBP1, CSBP2, CSPB1, EXIP, MAP Kinase MXI2, MAX-interacting Protein 2, Mitogen-acti

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian ortholog of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38 alpha, beta, gamma (also known as ERK6 or SAPK3) and delta (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3, MKK6 and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M2352-02C

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Rouse, J., et al., Cell 78: 1027-1037 (1994). 2. Han, J., et al., Science 265: 808-811 (1994). 3. Lee, J.C., et al., Nature 372: 739-746 (1994). 4. Freshney, N.W., et al., Cell 78: 1039-1049 (1994). 5. Raingeaud, J., et al., J. Biol. Chem. 270: 7420-7426 (1995). 6. Zervos, A.S., et al., Proc. Natl. Acad. Sci. USA 92: 10,531-10,534 (1995). 7. Zhao, M., et al., Mol. Cell. Biol. 19: 21-30 (1999). 8. Yang, S.H., et al., Mol. Cell. Biol. 19: 4028-4038 (1999).

Additional Info

Recognizes endogenous levels of total human p38a MAPK protein. Does not crossreact with other p38 MAPK isoforms such as beta, gamma or delta. Species Crossreactivity: mouse, rat and monkey.

Alternative Names

EC=2.7.11.24