MAP Kinase, p38, phosphorylated (Thr180/Tyr182) (MapK14, Mitogen-activated Protein Kinase p38 alpha, Mitogen-activated Protein Kinase 14, CRK1, CSBP1, CSBP2)

The p38 (CSBP, RK, or Mpk2) protein is the newest member of the MAP kinase family to be isolated. This protein was originally identified as a kinase phosphorylated on tyrosine in response to treatment of cells with LPS2. Subsequently, p38\n (CSBP1/CSBP2) was identified as the target of pyridinyl-imidazole compounds, which inhibit the production of interleukin-1 and tumor necrosis factor from stimulated human monocytes3. p38 and JNK appear to be activated by many of the same stimuli; nevertheless, differences in the extent and timing of activation of p38 and JNK have been observed4. p38 and JNK are activated by distinct MAPK kinases: MKK4 activates both JNK and p38, whereas MKK3 and MKK6 activate p38 specifically. Studies have implicated p38 in the phosphorylation of the small heat shock proteins Hsp27, MAPKAP kinase -\n2 and -3, and the transcription factors ATF2, Elk-1 and CHOP7. In addition, p38 likely mediates increased cytokine expression and some aspects of programmed cell death. Phosphorylation of p38 MAPK associated with the activation of NFkB may be related to human crescentic glomerulanephritis.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunohistochemistry: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

M2352-05D

Size

100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM HEPES, p.H 7.5, 150mM NaCl, 0.1mg/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by Protein A chromatography

References

1. Davis RJ. Trends Biochem Sci 19:470-473 (1994). 2. Han J, et al. Science 265:808-811 (1994) .3. Lee JC, et al. Nature 372:739-746 (1994) .4. Raingeaud J, et al. J Biol Chem 270:7420-7426 (1995). 5. Derijard B, et al. Science 267:687 (1995). 6. Raingeaud J, et al. Mol Cell Biol 16:1247-1255 (1996). 7. Freshney NW, et al. Cell 78:1039-1049 (1994). 8. Sakai N, et al. Nephrol Dial Transplant 17(6):998-1004 (2002).

Additional Info

Specific for 39kD phospho-p38 MAP Kinase, phosphorylated at Thr180/Tyr182. Immunolabeling in Western blots of C-6 glioma cells prepared with anisomycin treatment is blocked by the phosphopeptide but not by the dephosphopeptide. Reactivity has been confirmed with C-6 glioma cells. Species sequence homology: Rat.

Alternative Names

EC=2.7.11.24