MAP Kinase, p44/42 (Erk1/Erk2, Extracellular Signal Regulated Kinase)

Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. MAP kinases are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1] or 183 and 185 of rat Erk2) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). Both kinases are known to weakly autophosphorylate on tyrosine.\n\nApplications: \nSuitable for use in Immunofluorescence, Immunocytochemistry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Flow Cytometry. Other applications not tested.\n\nRecommended Dilution: \nWestern Blot: 1:1000; Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunohistochemistry(Paraffin sections): 1:100\nImmunoprecipitation: 1:50\nImmunocytochemistry (IF): 1:25\nFlow Cytometry: 1:25\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M2360-02

Size

200ul

Applications

ELISA, FC, ICC, IF, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

P Type

Pab

Purity

Purified by Protein A and immunoaffinity chromatography.

Isotype

IgG

References

1. Marshall, C.J., Cell 80: 179-185 (1995). 2. Hunter, T., Cell 80: 225-236 (1995). 3. Hill, C.S. and Treisman, R., Cell 80: 199-211 (1995). 4. Cowley, S., et al., Cell 77: 841-852 (1994). 5.

Additional Info

Recognizes endogenous levels of total human p44/42 MAP kinase (Erk1/Erk2) protein. In some cell types, recognizes p42 MAPK more readily than p44 MAPK. Does not recognize JNK/SAPK nor p38 MAP kinase. Species Crossreactivity: Human, mouse, rat, hamster, zebrafish.

Alternative Names

EC=2.7.11.24