MAP Kinase p44/42 (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, Mapk, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Kinase 2, MAPK 2, ERK2, PRKM1 /

Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. MAP kinases are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1] or 183 and 185 of rat Erk2) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). Both kinases are known to weakly autophosphorylate on tyrosine.\n\nApplications:\nSuitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot (Colorimetric): 1:1000\nFlow Cytometry: 10ug/ml \nImmunohistochemistry (Paraffin): 1:50\nOptimal dilutions to be determined by researcher.\n \nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M2360-02F

Size

50ug

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Ch/Bird, Dro/Arth, Sheep, Xen/Amph

Form

Supplied as a liquid.

P Type

Pab

Purity

Purified by Protein A affinity chromatography

Isotype

IgG

References

1. Dendritic Cell Differentiation Induced by a Self-Peptide Derived from Apolipoprotein E B. Singh, et al. J. Immunol. 181 6859 (2008). 2. Reduction of chronic allograft nephropathy by inhibition of extracellular signal-regulated kinase 1 and 2 signaling C. Du, et al. Am. J. Physiol. Renal Physiol. 295 F672 (2008). 3. Surface cytotoxic T lymphocyte-associated antigen 4 partitions within lipid rafts and relocates to the immunological synapse under conditions of inhibition of T cell activation J. Madrenas, et al. J. Exp. Med. 195 1337 (2002). 4. Stress-induced inhibition of ERK1 and ERK2 by direct interaction with p38 MAP kinase S. Pelech, et al. J. Biol. Chem. 276 6905 (2001). 5. Zap-70-independent Ca(2+) mobilization and Erk activation in Jurkat T cells in response to T-cell antigen receptor ligation R.L. Wange, et al. Mol. Cell. Biol. 21 7137 (2001)

Additional Info

Recognizes rat Erk1 at ~44KD. Species Crossreactivity: human, mouse, bovine, sheep, chicken, Drosophilia and Xenopus.

Alternative Names

EC=2.7.11.24