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MAP Kinase p44/42, phosphorylated (Thr202) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Kinase 2, MA

Cat no: M2352-20P

MAP Kinase p44/42, phosphorylated (Thr202) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Kinase 2, MA

Human p44/42 MAP Kinase belongs to the family of signal transduction intermediates leading to cell proliferation or differentiation, known as MAPKs (mitogen-activated protein kinases). It plays an important role in the signal transduction pathways that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals.Activation of the protein requires phosphorylation of both tyrosine and threonine residues by upstream dual-specific kinases. Active MAPKs are responsible for the phosphorylation of a variety of effector proteins, including several transcription factors.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\nImmunohistochemistry (paraffin): 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMCF7, 293, A431, A2780 and Hela cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M2352-20P

Size

50ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Letteria, M. et al. Biology of Reproduction. 73:730-736 (2005).

Additional Info

Recognizes human MAP Kinase p44/42, phosphorylated at Thr202. Species Crossreactivity: mouse and rat.

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