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MAP Kinase p44/42, phosphorylated (Thr202, Tyr204) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Kina

Cat no: M2352-15B

MAP Kinase p44/42, phosphorylated (Thr202, Tyr204) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Kina

Extra cellular signal-regulated kinases Erk1 (p44 MAPK) and Erk2 (p42 MAPK) Serine/Threonine proteine kinase are part of a subfamily of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth, differentiation, apoptosis and signal transduction (1,2,3) The simultaneous tyrosine/threonine phosphorylation of ERK1 and ERK2 results in their activation. Although ERK1and ERK2 resemble one another closely in sequence and are activated by similar pathways, they appearto have different tissue distributions(4) and show differential phosphorylation of potential intracellular substrates(5) \n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500\nImmunohistochemistry: 1:50\nImmunocytochemistry: 1:250\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

M2352-15B

Size

100ul

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Charest, D.L, et al.. Mol Cell Biol. 13: 4679

Additional Info

Recognizes human ERK1 phosphorylated on Threonine 202 and Tyrosine 204, or ERK2 phosphorylated on Threonine 185 and Tyrosine 187.

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