MAP1LC3B (LC3, Autophagy Related Protein LC3B, Autophagy Related Ubiquitin-like Modifier LC3B, MAP1 Light Chain 3-like Protein 2, Microtubule Associated Proteins 1A/1B Light Chain 3B, MAP1A/1B Light Chain 3B, MAP1A/MAP1B LC3B, Microtubule Associated Prote

LC3, a mammalian homologue of Apg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. However, LC3 is now thought to also be involved in autophagy. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. LC3-II is the first mammalian protein identified that specifically associates with the autophagosome membranes.\n\nCellular Localization: LC3-I is cytoplasmic. LC3-II binds to the autophagic membranes.\n\nPositive Control: Human/mouse brain lysate, ES, HeLa, HEK293, MCF7 cells, human lymphoblasts, and embryoid bodies\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry (paraffin-embedded sections). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L1564-50

Size

100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, ZF/Fish

Form

Supplied as a liquid in Tris-glycine, 150mM sodium chloride, pH 7.2.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Mizushimi, N., et al. Dissection of autophagosome formation using Apg5- deficient mouse embryonic stem cells. J. Cell Biol. 152:657-668 (2001)

Additional Info

This antibody is specific for LC3. Species Crossreactivity: This antibody reacts with human and mouse LC3 protein, though the mouse detection is weaker than the human. The immunogen used for this antibody production has 100% homology with rat and Zebrafish, 92% with mouse, 91% with bovine, and 84% with Xenopus proteins.