MARCKS (K152) pAb

Myristoylated alanine-rich protein kinase C substrate (MARCKS), also designated 80K or 80K-L, has been identified as a major cellular substrate for protein kinase C. Human MARCKS is a 332 amino acid protein with a calculated molecular weight of 31.534 kDa; however, it has been shown to run at 80-87 kDa on Western blot. The plasma membrane bound protein dissociates from the membrane upon phosphorylation by various PKC isoforms. In NIH/3T3 fibroblasts, PKC (alpha) and PKC E, but not PKC (delta), are responsible for MARCKS phosphorylation. MARCKS has been found to bind calmodulin, Actin and Synapsin and is a filamentous (F) Actin crosslinking protein.

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SPECIFICATIONS

Catalog Number

BS2982

Size

100ug/100ul

Applications

IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 125-175 of Human MARCKS.

Species

MARCKS (K152) pAb detects endogenous levels of MARCKS protein.

Dilutions

WB: 1:500~1:1000\nIF: 1:50~1:200

Swiss Prot

P29966

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul