Matrix Metalloproteinase 9, 92kD (MMP-9, Collagenase 4, Gelatinase B)

MMPs are a group of enzymes involved in matrix degradation. They share some important characteristics, such as a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and inhibition by specific proteinase inhibitors known as tissue inhibitors of metalloproteinases (TIMPs).The matrix metalloproteinases each have different substrate specificities within the ECM and are important in its degradation.. \n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Inhibition of MMP-9 activity. Other applications have not been tested. \n\nRecommended Dilutions: \nWestern Blot: 1ug/ml for 2hrs at RT under non-reducing conditions.\nImmunohistochemistry (Acetone-fixed frozen): 1:100 for 30 minutes at RT.\nImmunoprecipitation: 2-5ug/mg protein lysate. Use Protein G.\nInhibition: 2-4ug/ml\nOptimal dilutions to be determined by the researcher. \n\nPositve Control: \nConditioned serum-free medium from (dexamethasone -treated) human fibrosarcoma HT-10801 or endothelial HUVEC 4 cells.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M2425-23

Size

100ug

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography

Isotype

IgG1

References

1. Nikkari, S.T. et al. . American Journal of Pathology 149(5): 1427

Additional Info

Recognizes human MMP-9 at 92kD and 86kD (non-reducing conditions) which are identified as pro (latent) and active forms of matrix metalloproteinase-9 (MMP-9; also known as 92kD and collagenase IV or gelatinase B). Reacts with the complex (~200kD) of MMP-9 and tissue inhibitor of metalloproteinases-1 (TIMP-1)1. Does not cross-react with pro and active forms of MMP-2.