Matrix Metalloproteinase 9, Hinge (MMP-9, MMP9, 92kD Gelatinase, 92kD Type IV Collagenase, CLG4B, Gelatinase B, GELB, MANDP2)

Matrix metalloproteinases (MMP) belong to a family of proteases that are essential for the breakdown of extracellular matrix. Thus they play an important role in apoptosis, and tumor cell growth, invasion, metastasis, as well as in angiogenesis and wound healing. All MMPs contain a common domain structure, which include a signal sequence, a propeptide, a catalytic domain and a hemopexin-like (Hpx) domain. MMP-2 and MMP-9, the two gelatinases, contain three additional repeats of a fibronectin type II-collagen. Gelatinases degrade basement membrane collagen. Studies on the zebrafish model have illuminated the function of MMP-2 on the remodeling, fin regeneration, and gastrulation.\n\nApplications:\nSuitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:5000-1:20,000\nWestern Blot: 1:500-1:1000\nImmunohistochemistry: 1:50-1:200\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: M2425-43P\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M2425-43

Size

250ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

ZF/Fish

Form

Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Woessner, JF. FASEB J. 1991; 5:2145-54. 2. Ito, A, et al. J Biol Chem. 1996. 271:14657-60. 3. Fowlkes, JL, et al. J Biol Chem. 1995. 270:27481-8. 4. Itoh, Y and Nagase, H. Essays in Biochemistry. 2002. 38:21-36. 5. Andreasen, E.A., et al. 2007; Toxicol. Sci. 95(1): 215-226. 6. Bai, S., et al. 2005; Matrix Biol. 24(4):247-260. 7. Coyle, R.C., et al. 2008; Exp. Cell Res. 314(10): 2150-2162.

Additional Info

Recognizes zebrafish MMP-9.