MBD1 (Methyl CpG Binding Protein 1, MeCP1, CXXC3, MeCP1 Complex, Methyl CpG Binding Domain Protein 1, Methyl CpG Binding Protein MBD1, Protein Containing Methyl CpG Binding Domain 1, PCM1, Regulator of Fibroblast Growth Factor 2 (FGF 2) Transcription, RFT

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (3). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (4).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M2469-02B

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG1

Additional Info

This monoclonal antibody can be used for detection of MBD1, isoform 1, a 605 amino acid protein. In HeLa, a 70kD band is observed. Species Crossreactivity: Human.

Alternative Names

Methyl CpG Binding Protein Splice Variant 1, Methyl CpG Binding Protein Splice Variant 2, Methyl CpG Binding Protein Splice Variant 3, Methyl CpG Binding Protein Splice Variant 4