MBD3 (Methyl CpG Binding Protein 3)

MBD-3 (methyl-CpG-binding domain protein # 3) is a 38-40kD member of the MBD family of transcriptional repressors. Through its MBD, and as part of a large complex of regulatory proteins, it binds methylated DNA, promoting gene silencing during S phase. Although it can homodimerize, it is only active when heterodimerized with MBD-2. Human MBD-3 is 291 amino acids (aa) in length, and contains an N-terminal methyl binding domain (aa's 1-72) and a C-terminal coiled-coil region (aa's 216-245) that may be involved in protein-protein interaction. Human MBD-3 shares 95% aa identity with mouse, rat, and canine MBD-3, with differences essentially limited to the C-terminal 25 amino acids.\n\nApplications: \nWestern Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human MBD-3. The detection limit for rhMBD-3 is approximately 5 ng/lane under non-reducing and reducing conditions. \nDirect ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human MBD-3. The detection limit for rhMBD-3 is approximately 0.5 ng/well.\nOptimal dilutions should be determined by each laboratory for each application.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M2475-51

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.

P Type

Pab

Purity

Purified by human MBD-3 affinity chromatography.

Isotype

IgG

Additional Info

Selected for its ability to recognize human MBD-3 in direct ELISAs and Western Blots.