MDR Protein (MRP1, Multidrug resistance-associated protein 1, ABCC1, ATP-binding cassette sub-family C member 1, Leukotriene C(4) transporter)

MRP1 (also known as ABCC, GS-X, ABC29) functions as a multispecific organic anion transporter, with (oxidized) glutathione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. It also transports drugs and other hydrophobic compounds in presence of glutathione.\n\nApplications: \nSuitable for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nFlow Cytometry: 1:20; cell permeabilization is required\nImmunocytochemistry: 1:20-1:50 on cytospin preparations\nImmunohistochemistry: Paraffin sections\nWestern Blot: 0.2-2ug/ml\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M2825-13A

Size

1ml

Applications

FC, ICC, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.7% BSA, 0.1% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. D.R. Hipfner, et al.; Cancer Res. 54, 5788 (1994) 2. K.C. Almquist, et al.; Cancer Res. 55, 102 (1995) 3. M. Filipits, et al.; Clin. Cancer Res. 2, 1231 (1996) 4. D.R. Hipfner, et al.; Cancer Res. 56, 3307 (1996) 5. D.R. Hipfner, et al.; Br. J. Cancer 78, 1134 (1998) 6. S.R. Wright, et al.; Clin. Cancer Res. 4, 2279 (1998) 7. M. Filipits, et al.; Anticancer Res. 19, 5043 (1999) 8. M. Filipits, et al.; Adv. Exp. Med. Biol. 457, 141 (1999) 9. Q. Mao, et al.; Biochim. Biophys. Acta 1461, 69 (1999) 10. V.V. Rao, et al.; PNAS 96, 3900 (1999) 11. R. Daoud, et al.; Biochemistry 39, 6094 (2000) 12. R. Daoud, et al.; Biochemistry 39, 15344 (2000)

Additional Info

Recognizes the linear internal epitope (S918SYSGDI924) of human MRP1 (multidrug resistance-associated protein 1), overexpressed in various human non-P-glycoprotein MDR tumor cell lines. Does not crossreact with human P-glycoprotein, MDR3 P-glycoprotein or human MRP2 (cMOAT) to MRP5, or mouse MRP gene products.