MKP-3 (Mitogen-activated Protein Kinase Phosphatase 3, MAP Kinase Phosphatase 3, MKP3, Dual Specificity Protein Phosphatase 6, DUSP6, Dual Specificity Protein Phosphatase PYST1, PYST1)

MKP-3 is expressed constitutively in human skin fibroblasts and dephosphorylates and inactivates MAP kinase in vitro and in vivo. MKP-3 displays very low activity towards the stress-activated protein kinases (SAPKs) or RK/p38 in vitro, indicating that these kinases are not physiological substrates for MKP-3. MKP-3 is thought to play a role in the dephosphorylation and inactivation of MAP kinases and are therefore likely to be important in the regulation of diverse cellular processes such as proliferation, differentiation, and apoptosis. For this reason it has been suggested that MAP kinase phosphatases may be tumor suppressors (1). MKP-3 is expressed in lung, heart, brain, and kidney, but not significantly in skeletal muscle or testis. In situ hybridization studies of MKP-3 in brain reveal enrichment within the CA1, CA3, and CA4 layers of the hippocampus. Observations indicate that MKP-3 is a novel dual-specificity phosphatase that displays a distinct tissue distribution, subcellular localization, and regulated expression, suggesting a unique function in controlling MAP kinase family members (2).\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500 \nImmunohistochemistry: 1:50-1:100 \nFlow Cytometry: 1:60 \nImmunoprecipitation: 1:40 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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