MLH1 (Human Mul L Homolog, Familial Nonpolyposis Colin Cancer Type 2, COCA2, Colon Cancer Type 2, DNA Mismatch Repair Protein Mlh1, FCC2, hMLH1, HNPCC, HNPCC2, MGC5172, MutL Homolog 1, MutL Homolog 1 Colon Cancer Nonpolyposis Type 2, MutL Protein Homolog

MLH1 is a DNA mismatch repair protein. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPCC). MSHS2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.\n\nCellular Localization: Nuclear\n\nPositive Control: Tonsil, colon carcinoma\n\nApplications: \nSuitable for use in Immunofluorescence, Immunohistochemistry (paraffin-embedded sections) and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M3949-61A

Size

6ml

Applications

ICC, IF, IHC

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

As reported

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

Machin et al. J Cutan Pathol 29 (7): 415, 2002

Additional Info

Recognizes the human and mouse MLH1 (80-85kDa. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPC). MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair. This antibody cross reacts with human, hamster and rat Species Crossreactivity: Thsi antibody is reactive against human, mouse, hamster and rat.