MLH1 (Human Mul L Homologue, Familial Nonpolyposis Colin Cancer Type 2, COCA2, Colon Cancer Type 2)

Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA \nsynthesis, is crucial to the maintenance of genomic integrity. MLH1 is the human homologue of the E. coli MMR gene mutL. MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting \nof MLH1 and PMS1 (MutL-alpha), PMS2 (MutL-beta) or MLH3 (MutL-gamme). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases and DNA \nligase (reviewed in 1). Inactivation of the MLH1 gene causes genome instability and predisposition to cancer (2-5). The MLH1 gene is frequently mutated in hereditary nonpolypo- \nsis colon cancer (HNPCC) (6). MLH1 also plays a role in meiotic recombination (7). \n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. \nImmunoprecipitation: 1:200 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M3949-60C

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, NHP

Form

Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 0.02% sodium azide and 50% glycerol.

P Type

Mab

Purity

Purified

Isotype

IgG

References

1. Modrich, P. (2006) J Biol Chem 281, 30305-9, 2. Seng, T.J. et al. (2008) Br J Cancer 99, 375-82, 3. Harley, I. et al. (2008) Gynecol Oncol 109, 384-7, 4. Mao, G. et al. (2008) J Biol Chem 283, 3211-6, 5. Hubner, R.A. and Houlston, R.S. (2007) J Natl Cancer Inst 99, 1490; author reply 1490-1, 6. Vasen, H.F. (2005) Fam Cancer 4, 219-25, 7. Argueso, J.L. et al. (2003) Mol Cell Biol 23, 873-86.

Additional Info

Recongnizes endogenous levels of human total MLH1 protein. Species crossreactivity: monkey.