MLH1 mAb

Cat no: MB0151

Supplier: Bioworld Technology, Inc.

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Heterodimerizes with PMS2 to form MutL alpha,a component of the post-replicative DNA mismatch repair system (MMR).DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex.Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2.It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch.DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected.MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III,suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR.Also implicated in DNA damage signaling,a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.

Catalogue number:	MB0151
Reactivities:	Human
Hosts:	Mouse
Applications:	Western Blot
Size:	100ug/100ul
Swiss prot:	P40692
Dilutions:	WB: 1:500~1000 ICC: 1:50~200
Form:	liquid
Storage buffer:	1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration:	1ug/ul
Antigen:	Recombinant full length Human MLH1.
Species:	This antibody detects endogenous levels of MLH1 and does not cross-react with related proteins
Molecular weight:	Predicted band size:85KDa Observed band size:85KDa
Purity:	The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp:	Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names:	COCA 2; COCA2; DNA mismatch repair protein Mlh1; FCC 2; FCC2; hMLH 1; hMLH1; HNPCC 2; HNPCC; HNPCC2; MGC5172; MLH 1; MLH1; MLH1; MutL homolog 1 (E. coli); MutL homolog 1; MutL homolog 1 colon cancer nonpolyposis type 2; MutL homolog 1,colon cancer,nonpolyposis type 2 (E. coli); MutL protein homolog 1; MutL,E.coli,homolog of,1; FCC2; COCA2; HNPCC; hMLH1; HNPCC2.
Additional info:	For research use only, not for use in diagnostic procedure.

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MLH1 mAb

Cat no: MB0151

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