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MLLC (HRX)

Cat no: M4127

MLLC (HRX)

Applications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 0.1-1ug/ml detects MLLC in nuclear extract from K562 cells. K562 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with M4127 (1ug/ml). Proteins were visualized using a goat anti-mouse (HRP) and a chemiluminescence detection system.\nImmunoprecipitation: Reported by an independent laboratory to immunoprecipitate MLLC from nuclear extracts. The N-terminal fragment, MLLN (300kD), is also co-immunoprecipitated by this antibody.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo\nI1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo\nI1904-14C: IgG, Fc (HRP) Pab Gt xMo\nI1904-19C: IgG, F(ab

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SPECIFICATIONS

Catalog Number

M4127

Size

200ug

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Yokoyama, A., et al., Blood 100: 3710-3718, 2002.

Additional Info

Recognizes the C-terminal proteolytic fragment of human MLL (MLLC) at~180kD. Species Crossreactivity: mouse

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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