Monocarboxylate Transporter 2 (MCT2)

Monocarboxylate such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end product of glycolysis. Some tissues, such as white skeletal muscle and, red blood cells, use this pathway to generate most of their ATP under normal physiological conditions. All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain maintain high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis. Some tissues, such as brain, heart, and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs). At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner. MCT2/MOT2 (mouse 484aa, rat 489aa, human 478aa, chromosome 12q13) is less widely distributed than MCT. It is associated with tissues that demonstrate a high uptake affinity for lactate and pyruvate such as the kidney and liver (for gluconeogenesis) and neurons (for oxidation). It may function in transporting lactate from intestine and erythrocytes. MCT1 is most closely related to MCT1 (~55% identity, whereas homolog with other MCT1-MCT8 isoforms is less (~35-53%). Both N and C-termini are predicted to be cytoplasmic.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well (Cat #M4470-12A)\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M4470-12B

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Jackson VN et al (1997) Biochem. J. 324, 447-453. 2. Dao L et al (1998) J. Biol. Chem. 273, 28959-28965. 3. Koehler-Stec EM et al (1998) Am. J. Physiol. 275, E516-E524. 4. Price NT et al (1998) Biochem. J. 329, 321-328. 5. Halestrap AP and Price NT (1999) Biochem J. 343, 281-299

Additional Info

Recognizes human MCT2. Human sequence is not well conserved in MCT2 from other species. No significant sequence homology exists with other MCTs.