MSH6 mAb

Component of the post-replicative DNA mismatch repair system (MMR).Heterodimerizes with MSH2 to form MutS alpha,which binds to DNA mismatches thereby initiating DNA repair.When bound,MutS alpha bends the DNA helix and shields approximately 20 base pairs,and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA.After mismatch binding,forms a ternary complex with the MutL alpha heterodimer,which is thought to be responsible for directing the downstream MMR events,including strand discrimination,excision,and resynthesis.ATP binding and hydrolysis play a pivotal role in mismatch repair functions.The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange,resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone.This transition is crucial for mismatch repair.MutS alpha may also play a role in DNA homologous recombination repair.

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SPECIFICATIONS

Catalog Number

MB0153

Size

100ug/100ul

Applications

WB

Hosts

Mouse

Reactivities

Hum

Form

liquid

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Recombinant full length Human MSH6.

Species

This antibody detects endogenous levels of MSH6 and does not cross-react with related proteins

Dilutions

WB: 1:300~500\nICC: 1:50~100

Swiss Prot

P52701

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

Predicted band size:160KDa\nObserved band size:160KDa

Alternative Names

DNA mismatch repair protein Msh6; G/T mismatch binding protein; G/T mismatch-binding protein; GTBP; GTMBP; hMSH6; HNPCC 5; HNPCC5; HSAP; MSH 6; MSH6; MSH6; mutS (E. coli) homolog 6; MutS alpha 160 kDa subunit; mutS homolog 6; MutS-alpha 160 kDa subunit; p160; Sperm associated protein; GTBP; HSAP; HNPCC5