mTOR (FK506 binding protein12-rapamycin associated protein 2, FKBP rapamycin associated protein, FKBP12 rapamycin complex associated protein, FLJ44809, FRAP)

Mammalian target of rapamycin (mTOR) is a serine and threonine protein kinase that regulates numerous cellular functions, in particular, the initiation of protein translation. Rapamycin is a natural product macrolide that induces G1 growth arrest in yeast, Drosophila, and mammalian cells. mTOR has a long list of synonyms including FK506 binding protein12-rapamycin associated protein 1, FK506 binding protein12-rapamycin associated protein 2, FRAP1, FRAP2, RAFT1, RAPT1 and/or FKBP12-rapamycin associated protein(FRAP). mTOR is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, mTOR is a 245kD protein referred to as RAFT1 with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of mTOR to FKBP12-rapamycin correlates with the ability of these ligands to inhibit cell cycle progression.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:4000-1:20,000\nWestern Blot: 1:250-1:2000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

168379

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human mTOR. Species Crossreactivity: mouse and rat.

Alternative Names

FK506 binding protein12-rapamycin associated protein 2 antibody, FKBP rapamycin associated protein antibody, FKBP12 rapamycin complex associated protein antibody, FLJ44809 antibody, FRAP antibody