Muc4 (Mucin-4, MUC-4, Ascites Sialoglycoprotein, ASGP, Pancreatic Adenocarcinoma Mucin, Pre-sialomucin Complex, pSMC, Sialomucin Complex, Testis Mucin, Smc)

MUC4 has been found to be a heterodimeric glycoprotein complex that consists of ASGP-1 (ascites sialoglycoprotein-1), an O-glycosylated mucin subunit, and ASGP-2, an N-glycosylated transmembrane subunit. ASGP-1 and ASGP-2 is a multifunctional glycoprotein complex. ASGP-1, through its O-glycosylation of the tandem repeat domain, provides anti-recognition and anti-adhesive properties to tumor cells. ASGP-2 has two epidermal growth factor-like domains. ASGP-2 appears to be able to bind and modulate ErbB2 receptor phosphorylation, which allows rapid growth of tumor cells. Sialomucin complex (SMC, rat homologue of human MUC4) expression is found in normal secretory epithelial tissues in the adult rat, where the protein has multiple regulatory mechanisms. SMC is found in normal virgin mammary gland, elevated in the lactating gland, and further amplified in rat mammary adenocarcinoma. Transforming growth factor-beta (TGF-b) inhibits SMC expression. Abnormal expression of MUC4/SMC is found in several human epithelial tumors such as, breast, lung, cervix, and pancreas carcinomas. Unregulated MUC4 may promote tumor metastasis, which suggests that it has an important role in human tumor biology. For example, several studies indicate that the cytoplasmic tail of the transmembrane mucin is involved in cell-signalling events such as Sos-ras pathway, beta-catenin, and ErbB2 signalling pathway.\n\nApplications:\nSutiable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot: 1-2ug/ml\nELISA (rat): <0.1ug/ml \nImmunohistochemistry (human): 2-5ug/ml. Heat-induced epitope retrieval (Citrate buffer, pH 6.0) \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M4700-89A

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide

P Type

Mab

Purity

Purified by Protein A chromatography

Isotype

IgG1

References

1. Carraway, K.L. et al; Cancer Control: 6 (6) 613-614 (1999) 2. Carraway, C.C. et al; J. Biol. Chem: 274 (36) 25659-25667 (1999). 3. Perrais, M, et al; J. Biol. Chem: 276 (33) 30923-30933 (2001). 4. Shari A, et al; J. Biol. Chem: 275 (23) 17800-17807 (2000). 5. Li, Y.Q. et al; J. Biol. Chem: 274 (36) 25651-25658 (1999).

Additional Info

Recognizes an epitope on the rat ASGP-2 subunit, which is one of the main subunits that comprise the MUC4 glycoprotein complex. Reactivity is confirmed with ASGP-2 transfected COS-7 cells and rat microvilli isolated from ascites cells of 13762 rat mammary adenocarcinoma. Species Crossreactivity: human and mouse.