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Mug, Recombinant, aa1-168, His-Tag (G/U Mismatch-specific DNA Glycosylase, Xanthine DNA glycosylase, Dug, ECK3058, JW3040, YgjF)

Cat no: 137127

Mug, Recombinant, aa1-168, His-Tag (G/U Mismatch-specific DNA Glycosylase, Xanthine DNA glycosylase, Dug, ECK3058, JW3040, YgjF)

G/U mismatch-specific DNA glycosylase, xanthine DNA glycosylase, also known as mug, belongs to the TDG/mug DNA glycosylase family. It has been proposed that the Mug protein excises 3, N4-ethenocytosine and removes the uracil base from mismatches in the order of U:G>U:A, although the biological role remains unclear. The enzyme Uracil-N-Glycosylase removes uracil from the DNA leaving an AP site. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. \n\nSource: \nRecombinant protein corresponding to E.coli mug, fused to His-tag at N-terminal expressed in E.coli.\n\nMolecular Weight: \n~21.1kD (191aa) (MALDI-TOF) \n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MGSMVEDILA PGLRVVFCGI NPGLSSAGTG FPFAHPANRF WKVIYQAGFT DRQLKPQEAQ HLLDYRCGVT KLVDRPTVQA NEVSKQELHA GGRKLIEKIE DYQPQALAIL GKQAYEQGFS QRGAQWGKQT LTIGSTQIWV LPNPSGLSRV SLEKLVEAYR ELDQALVVRG R\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

137127

Size

50ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.1M NaCl, 20% glycerol.

Purity

~90% (SDS-PAGE)

References

1. Lee HW., et al. (2010) J Biol Chem. 285(53):41483-90. 2. Gallinari P., et al. (1996) Nature. 383(6602):735-8.

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Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

More info
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