mutY, Human Control Peptide

Control Peptide for M9555-01 (serum) and M9555-01 (affinity purified).\n\nEscherichia coli MutY is an adenine DNA glycosylase active on DNA substrates containing A/G, A/8-oxoG, or A/C mismatches and also has a weak guanine glycosylase activity on G/8-oxoG-containing DNA. The N-terminal domain of MutY, residues 1-226, has been shown to retain catalytic activity. The post-replicative adenine:8-oxo-7,8-dihydroguanine (GO) mismatch is crucial for G:C to T:A transversion. This mismatch is corrected by Escherichia coli MutY which excises the adenine from A:GO. A candidate gene coding for the human counterpart of MutY has been cloned as hMYH (535 aa) (1)\n\nApplications:\nSuitable for use in ELISA or Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.\n\nRecommended Dilutions:\nAntibody Blocking: 5-10ug per 1ul M9555-02 (serum) or per 1ug M9555-03 (affinity purified antibody).\nELISA: Coat plates wtih 50-100ng/ml.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M9555-01

Size

50ug

Applications

ELISA, WB

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

Purity

Highly purified.

References

1.Slupska MM et al., J. Bacteriol. 178:3885-3892 (1996). 2.Slupska MM et al., J. Bacteriol 181:6210-3 (1999) 3. Ohtsubo T, et al., Nucleic Acids Res 15:1355-1364 (2000). 4. Wright PM et al., J Biol Chem 27,:29011-29018(1999) 5.Takao M et al., Nucleic Acids Res. 27: 3638-44(1999). 6. Noll DM et al., Biochemistry 38:6374-9(1999)

Additional Info

A 19-aa synthetic peptide near the C-terminus of human mutY. No significant homology with E. coli mutY (1).