Myeloperoxidase, Fluorometric Activity BioAssay(TM) Kit (MPO, pANCA)

Myeloperoxidase (MPO) is a peroxidase enzyme (EC 1.11.1.7) most abundantly present in neutrophil granulocytes. It is a green hemoprotein found in neutrophils and monocytes that catalyzes the reaction of hydrogen peroxide and halide ions to form cytotoxic acids and other intermediates that play a role in the oxygen-dependent killing of tumor cells and microorganisms. Its heme pigment causes the green color in secretions rich in neutrophils, such as pus and some forms of mucus. Furthermore, it can oxidize tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent. MPO catalyzes the production of NaClO from H?O? and NaCl. Subsequently, NaClO will react stoichiometrically with Aminophenyl fluorescein (APF) to generate fluorescein, which has a strong fluorescence and can be detected at Ex/EmH5/525nm. The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput assay of MPO activity. This kit can be used to detect MPO activity as low as 0.5uU per well.\n\nSample Type:\nCell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids etc.\n\nIntended Use:\nThe kit provides a convenient means for detecting formate in biological samples such as in serum or plasma, cells, culture and fermentation media.\n\nSensitivity:\n0.5uU per well\n\nRange:\n0.5-10nmols/well\n\nTest Principle:\nIn the Formate Assay Kit, formate is oxidized to generate a product resulting in color formation (l=450nm) proportional to formate concentration. The kit provides a convenient means for detecting formate in biological samples such as in serum or plasma, cells, culture and fermentation media. There is no need for pretreatment or purification of samples.\n\nSpecificity:\nMammalian\n\nKit Components:\nAssay Buffer: 1x25ml\nMPO Substrate Stock: 1x50ul\nMPO Probe: 1x200ul\nFluorescein Standard (1 mM): 1x50ul\nMPO Positive Control: 1x1vial\n\nStorage and Stability:\nStore components at 4 degrees C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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