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Myeloperoxidase (MPO)

Cat no: 136273

Myeloperoxidase (MPO)

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains (12 kD) and 2 heavy chains (60 kD). This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. It is released in a degranulation process in response to bursts of oxygen consumption, which also forms H2O2. MPO and H2O2 form a complex that oxidases a large variety of substances some of which have microbicidal effects. The gene for MPO is translocated in patients with acute promyelocytic leukemia and is mutated in hereditary MPO deficiency.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.\n\nRecommended Dilution:\nWestern Blot: 1:2000 \nImmunohistochemistry (Paraffin): 1:200\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

136273

Size

50ul

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.

P Type

Pab

Purity

Purified

Isotype

IgG

Additional Info

Recognizes human MPO.

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SUPPLIER INFO

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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