Myeloperoxidase, Recombinant, Mouse (MPO, Myeloperoxidase Precursor)

Mouse Myeloperoxidase (MPO) is a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that catalyzes the hydrogen peroxide-dependent conversion of chloride, bromide, and iodide to multiple reactive species. Post-translational processing of human MPO involves the insertion of a heme moiety and the proteolytic removal of both a propeptide and a 6 aa internal peptide. This results in a disulfide-linked dimer composed of a 60kD heavy and 12kD light chain that associate into a 150kD enzymatically active tetramer. The tetramer contains two heme groups and one disulfide bond between the heavy chains. Mouse and human MPO share 87% aa sequence identity. MPO activity results in protein nitrosylation and the formation of 3-chlorotyrosine and dityrosine crosslinks. Modification of ApoB100, as well as the lipid and cholesterol components of LDL and HDL, promotes the development of atherosclerosis. MPO is also associated with a variety of other diseases, and inhibits vasodilation in inflammation by depleting the levels of NO. Serum albumin functions as a carrier protein during MPO movement to the basolateral side of epithelial cells. MPO is stored in neutrophil azurophilic granules. Upon cellular activation, it is deposited into pathogen- containing phagosomes. While mice lacking MPO are impaired in clearing select microbial infections, MPO deficiency in humans does not necessarily result in heightened susceptibility to infections.\n \nSource: \nDNA sequence encoding mouse Myeloperoxidase (Met16-Thr 718; AAR99349) fused with a C-terminal 10-His tag expressed in NS0 cells. \n\nN-terminal Sequence Analysis:\nMet16\n\nSDS-PAGE:\n85-95kD, reducing conditions\n \nActivity: \nMeasured by its ability to oxidize guaiacol in the presence of hydrogen peroxide (15).\n\nSpecific Activity:\n> 8000 pmoles/min/ug under the described conditions. See protocol.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M9760-10L

Size

250ug

Form

Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with sterile ddH2O to 0.5mg/ml.

Purity

(same/more than)95% by SDS

References

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