MYH pAb

MUTYH (mutY homolog (E. coli)) is a DNA glycosylase mismatch repair enzyme that in conjunction with mutM (OGG1), cleaves adenine residues paired with either oxidized (8-hydroxyguanines) or non-modified guanines in order to correct A/G and A/C mismatches. Repair of most modified and mispaired bases in the genome is initiated by DNA glycosylases, which bind and cleave N-glycosyl bonds to initiate base excision repair. MUTYH is crucial for the avoidance of mutations resulting from oxidative DNA damage. Multiple N-terminal splice variants of MUTYH exist in mammalian cells. Increasing MUTYH in A549 cells exposed to oxygen and infrared radiation leads to improvements in cell survival. Biallelic MUTYH germ-line mutations predispose humans to colorectal adenomas and carcinomas. MUTYH is abundant in neurons where mitochondrial genomes exposed to reactive oxygen species (ROS) that damage DNA must maintain integrity over the entire mammalian life span.

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SPECIFICATIONS

Catalog Number

BS6673

Size

100ug/100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Recombinant full length Human MYH.

Species

MYH pAb detects endogenous levels of MYH protein.

Dilutions

WB: 1:500~1:2000\nIHC: 1:50~1:200

Swiss Prot

Q9UIF7

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 60 kDa

Alternative Names

A/G specific adenine DNA glycosylase; A/G-specific adenine DNA glycosylase; Adenine DNA glycosylase; CYP2C; hMYH; MGC4416; MutY (E. coli) homolog; MutY homolog (E. coli); MutY homolog; Mutyh; MUTYH; MYH; rMYH;