Myoglobin, Human, BioAssay(TM) ELISA Kit

The United States Biological Myoglobin, Human, BioAssay(TM) ELISA Kit is intended for the quantitative determination of myoglobin in human serum.\n\nMyoglobin, a heme protein with a molecular weight of approximately 17.5kD is found in both cardiac and skeletal muscle. Damage to either type of muscle following conditions such as trauma, ischemia, and diseases that cause myopathy, is associated with the release of myoglobin into serum. Specifically, following cardiac necrosis associated with myocardial infarction (MI), myoglobin is one of the first markers to rise above normal levels. Myoglobin levels increase measurably above baseline within 2-4 hours post-infarct, peaking at 9-12 hours, and returning to baseline within 24-36 hours. In the absence of skeletal muscle trauma or other factors associated with a non-cardiac related increase in circulating myoglobin, its levels have been used as an early marker for myocardial infarct. A number of reports suggest using the measurement of myoglobin as a diagnostic aid in ruling out myocardial infarction with negative predictive values of up to 100% reported at certain time periods after the onset of symptoms. Unlike the other cardiac enzymes such as creeatine kinase and the MB isoform (i.e., CK and CK/MB) which do not reach serum levels until several hours post- infarction (~19 hours), myoglobin levels can be expected to peak within 6-9 hours. \n\nThe Myoglobin, Human, BioAssay(TM) ELISA Kit provides a rapid, sensitive, and reliable assay for the quantitative measurement of myoglobin in serum. The antibodies developed for the test will determine a minimal concentration of 5.0ng/ml, and there is no cross-reactivity with related cardiac or skeletal enzymes. \n\nRange: 0-1000ng/ml\n\nSensitivity: 5ng/ml\n\nSpecificity: ~95%\n\nKit Components:\nM9800-05A: Microtiter Plate, 1x96wells\nM9800-05B: Standard 0ng/ml, 1x500ul \nM9800-05C: Standard 25ng/ml, 1x500ul \nM9800-05D: Standard 100ng/ml, 1x500ul \nM9800-05E: Standard 250ng/ml, 1x500ul \nM9800-05F: Standard 500ng/ml, 1x500ul \nM9800-05G: Standard 1000ng/ml, 1x500ul \nM9800-05H: Sample Diluent, 1x25ml \nM9800-05J: Pab (HRP), 1x22ml \nM9800-05K: TMB Solution, 1x11ml\nM9800-05L: Stop Solution (1N HCl), 1x11ml \n \nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M9800-05

Size

1Kit

Applications

ELISA

Reactivities

Hum

References

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