Myosin, Skeletal Muscle, heavy chain

Myosin is a highly conserved, ubiquitous protein found in all eukaryotic cells, where it provides the motor function for diverse movements such as cytokinesis, phagocytosis, and muscle contraction. All myosins contain an amino-terminal motor/head domain and a carboxy-terminal tail domain. Based on the properties of the head domain, myosin is divided into seven distinct classes. Class II myosin, one of the widely studied myosin consists of the conventional two-headed myosins that form filaments and are composed of two myosin heavy chain (MYH) subunits and four myosin light chain subunits. The MYH subunit contains the ATPase activity providing energy that is the driving force for contractile processes. Numerous MYH isoforms exist in vertebrates to carry out this function. In humans, the skeletal myosin heavy chain (MYH) gene is clustered on a single chromosome (17p).\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 1:25-1:50\nImmunohistochemistry: Frozen\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nSkeletal muscle\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M9850-15H

Size

500ul

Applications

IHC

Hosts

Mouse

Reactivities

Rat, Bov, Ch/Bird, GP, Rabbit

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. Yoon et al. Proc Natl Acad Sci USA 89: 12078, 1992. 2. Weiss et al. Annu Rev Cell Dev Biol 12: 417, 1996.

Additional Info

Recognizes heavy chain of skeletal myosin. It does not stain cardiac or smooth muscle myosin. This antibody stains the fast twitch (type II) isomyosin molecule. Species Crossreactivity: bovine, rabbit, chicken, guinea pig, rat.