Myosin, Smooth Muscle, Heavy Chain 2 (MHC-2)

The heavy chain of myosin II found in vertebrate smooth muscle is a non-striated muscle present in the walls of hollow organs, the vasculature, the respiratory and gastrointestinal tracts, uterus, bladder and eye.\n\nThe structure of myosin II is fundamentally the same in all muscle types and consists of two heavy chains, each bound by 2 distinct, essential and regulatory light chains. The globular motor domain located at the N-terminal domain of the heavy chains, catalyzes ATP hydrolysis and interacts with actin, whilst the heptad repeat sequences of the heavy chain tail domains promote dimerization, forming an alpha-helical coiled coil at the C-terminal domain.\n\nApplications:\nSuitable for use in Immunofluorescence, Immunohistochemistry (frozen), and Western Blot. Other applications not tested. \n\nRecommended Dilution:\nWestern Blot: 1:150\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M9850-12C

Size

500ul

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Bov

Form

Supplied as a liquid in 20mM TRIS, pH 7.5, 150mM sodium chloride, 0.006% sodium azide.

P Type

Pab

Purity

Purified by Ion Exchange chromatography.

Isotype

IgG

References

1. Rosenfeld, S.S. et al. (1998) Structural and kinetic studies of phosphorylation-dependent regulation in smooth muscle myosin. J. Biol. Chem. 273: 28682-28690.

Additional Info

Recognizes the heavy chain of myosin II found in vertebrate smooth muscle. Does not crossreact with myosin of skeletal or non-muscle origin. Species Crossreactivity: vertebrates.