Myosin, Smooth Muscle Heavy Chain (BSA & Azide Free)

SM-MHC is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmently regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. \n\nApplications: \nSuitable for use in Immunohistochemistry. Other applications have not been tested. \n\nRecommended Dilutions: \nImmunohistochemistry (Formalin/paraffin): 1:25 for 60 minutes at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes. followed by cooling at RT for 20 minutes. \nOptimal dilutions to be determined by the researcher. \n\nPositive Control: \nUterus \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M9850-16X

Size

100ul

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4. Also available with BSA and sodium azide. See M9850-16.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Frid, M.G., et al., Dev. Biol. 153: 185 (1992). 2. Glukhova, M.A., et al., J. Biol. Chem. 265(22): 13,042 (1990).

Additional Info

Recognizes human MHC-1 (205kD) and MHC-2 (200kD) isoform, staining the intact myoepithelial cell layers present in benign and in situ malignant breast lesions.